Post Doc - Open Rank

Collaborates with team members to develop and refine genome editing strategies for in vivo editing and immune modulation using CAR Tregs.
• Designs and executes experiments to evaluate the safety and efficacy of genome-edited cells.
• Assesses the functionality of engineered cells,and evaluates their therapeutic potential both in vitro and in vivo that includes designing CRISPR-Cas targeting strategies, analyzes editing outcomes on a molecular level using next gen sequencing and ddPCR and conducts functional assays such as cytotoxicity assays, ELISPOT, ELISA and cytokine release assays.
• Design and execute experiments in mouse models and assess efficacy of therapeutics.
• Perform histological analysis to perform phenotypic assessment of editing outcomes
• Analyze experimental data and interprets results.
• Apply statistical methods to analyze data from experiments, that includes next-generation sequencing data, flow cytometry data, and functional assay results.
• Communicates findings effectively through presentations in lab meetings and international conferences and scientific publications
• Contributes to the preparation of grant proposals and scientific publications.

Interested applicants should write a cover letter explaining their interest in the research project, copy of their CV and contact information of three references and send their applications to Dr Allison Keeler. Allison.Keeler@umassmed.edu

• Ph.D. in Basic Science, Health Science, or a related field with an interest in working in translational science.
• Experience in cell culture, basic molecular biology skills, immunology assays, gene editing and gene therapy is preferred.
• Experience with designing mouse experiments is desirable.